The oral exam will be on the last day of 3-weeks period.
It is an individual oral exam taking 20-25 minutes per student and graded according to the 7-step scale.
The layout of the exam is:
• Present an argued purification strategy and the obtained results (TPH or FXD) - 10 min. (powerpoint or on paper)
• Draw one set of questions to be answered without aids - 10 min.
(writing board, blank paper, and writing utensils will be available)
Questions are made available the first day of course.
• General discussion of the subject - 5 min.
• Grading - 5 min.
The chromatographic system ÄKTA Start and columns will be available for the oral exam.
Questions
1: Describe the principle of IEX
Describe the concepts of choosing an appropriate buffer system and pH for IEX
2: Describe the principle of IEX
Discuss the pros and cons of column materials with different particle size distribution
3: Describe the principle of GF
Discuss the pros and cons of using GF in the different steps of a purification strategy
4: Describe the principles of HIC
Describe and discuss methods for extraction of soluble proteins from solid samples (cells)
5: Describe the four most important factors for chromatographic purification and discuss when the individual factors are most important in a purification strategy
6: Describe the principles of AC
Describe the concept and discuss the pros and cons of using purification-tags
7: Name and describe the function of the different components of the ÄKTA start system
What do you need to know about a protein in order to devise a purification strategy and why?