Preparation of the Pyrococcus furiosus ferredoxin purification
This is an example of a typical protein purification protocol. It consists of three column chromatographic steps and several in between steps. You will spend approximately three days on this purification. The most important and critical factor for a successful result is overview and preparation. Therefore, this module is allocated to prepare for the purification ahead.
Please do NOT underestimate the time required to prepare. The protocol can be found here.