DTU SCore: Automated HTS to accelerate biochemical and cellular research

The fully automated DTU Screening Core provides state-of-the-art facilities for medium and high throughput screening at DTU Chemistry.
Biochemical assay performed in our screening facility

Services

The state-of-the-art facility is designed to perform biochemical, biophysical, and cell-based screenings in 96-, 384- and 1,536-well format. It offers great flexibility in terms of possible read-outs, such as fluorescence, absorbance, luminescence and imaging function among others, thanks to the Tecan Spark Cyto 600 multimode microplate reader with cell-imaging function.

  • Assay development
  • Assay adaptation and miniaturization in HTS format
  • Pilot screening
  • Full library screening
  • Focused subset library screening
  • Hit confirmation
  • Potency determination
  • Data analysis
  • Compound toxicity in cell viability assays
  • Assays in 96-, 384- and 1536-well format
  • 50,000 compounds from the DK-OPENSCREEN library
  • Hit identification and data analysis 
  • Assay readouts:
    • Absorbance UV/Vis 
    • Fluorescence
    • Fluorescence polarization
    • Luminescence
    • Alpha technology
    • Imaging (Fluorescence, Bright field, Digital phase contrast)

The facility offers screenings at different levels. Services include assay development, miniaturization, HTS adaptation, and pilot or full library screening. In addition, we offer data analysis and hit validation as well as plate printing of our in-house compound collection or the user’s own compound library.

DTU Screening Core facility works in close collaboration with DK-OPENSCREEN, the national research infrastructure for chemical biology, which offers their compound collection, consisting of 50,000+ compounds, in an open-access form. Production of assay ready plates with the in-house compound collection is part of the activities of the platform and is handled by the acoustic liquid dispenser ECHO 550. 

The platform is used by both academic groups and industry.

Instrumentation

  • Spark CYTO 600, TECAN – Multimode microplate reader
  • ECHO 550, LabCyte – Acoustic liquid handling
  • MultiFlo FX, BioTek - Multi-Mode Dispenser
  • EL406, BioTek - Washer Dispenser
  • CyBio FeliX, Analytik Jena - Pipetting Robot Liquid Handling
  • Cell culture facility

Compound collection

The compound collection provided by DK-OPENSCREEN gives access to a library consisting of 50,000 diverse and lead-like compounds for HTS rationally selected from different commercial vendors aiming to span multiple assays and targets.

DK-OPENSCREEN also offer ~2000 academic compounds and ~700 fluorinated fragments. 

Cherry picked compounds from our compound library.
DTU Screening core facility

Collaboration

We are pleased to work in collaborative projects with internal DTU partners as well as external partners. We are also happy to support your grant applications with letters and our technical information.

Contact

For any technical enquiries concerning assays, collaborations, prices, timelines and availability, please contact us at dtuscore@dtu.dk 
Mads Hartvig Clausen

Mads Hartvig Clausen Professor Department of Chemistry Phone: +45 45252131 Mobile: +45 20840131

Faranak Nami

Faranak Nami Platform Manager Department of Chemistry Mobile: +45 22792357

Luca Laraia

Luca Laraia Professor Department of Chemistry Phone: +45 45252495 Mobile: +45 50652777