Metalloprotein Design and Engineering

Rational metalloprotein design is a novel approach for testing general theories of metalloprotein chemistry through the creation of new structures and functions. It has further perspectives in the generation of new catalytic systems. The main focus of our research is on iron-sulfur proteins. These proteins are widespread in nature, and function both as electron transfer proteins and as catalytic proteins (enzymes). We have designed, synthesized and characterized both mini-rubredoxins and mini-ferredoxins. Current effort is on active site redesign of [4Fe-4S] iron-sulfur proteins through the incorporation of non-natural catalytically relevant active sites. A wide range of techniques encompassing recombinant DNA technology, inorganic synthesis, electrochemistry and spectroscopy are employed.



If you have any questions or comments on this research programme please contact Hans E. Mølager Christensen, hemc@kemi.dtu.dk