Gel Filtration Chromatography - concepts

The overall principle of gel filtration chromatography is outlined in this video:
The Principle of Gel Filtration
Gel filtration (also known as size exclusion chromatography (SEC)) be divided into two main areas:

1) High-resolution purification of biomolecules

2) Group separations

To get working knowledge of gel filtration for purification of proteins please read and digest the following chapters in the handbook 'Size Exclusion Chromatography'. Introduction (page 7-8), chapter 1, chapter 2, chapter 5, and chapter 7.